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rabbit polyclonal anti atf6 (Proteintech)

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Proteintech rabbit polyclonal anti atf6
Rabbit Polyclonal Anti Atf6, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 452 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/atf6+polyclonal+antibody/ATF6+Antibody/pmc12866116-14-0-7
Average 96 stars, based on 452 article reviews

rabbit polyclonal anti atf6 - by Bioz Stars, 2026-09

96/100 stars

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Article Title: α-Lipoic acid protects HAECs from high glucose-induced apoptosis via decreased oxidative stress, ER stress and mitochondrial injury
Article Snippet: a-Lipoic acid (LA) has a wide range of benefits in treating diabetes mellitus (DM) and DM vascular diseases, however, the specific mechanisms are not clearly understood.. The purpose of this study was to investigate whether LA confers a cytoprotective effect on human aortic endothelial cells (HAECs) in the presence of high glucose concentration and the possible mechanisms involved in this effect.. The apoptotic cells were detected by Annexin-V/PI and DAPI staining.

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Article Title: Targeting ceramide transfer protein sensitizes AML to FLT3 inhibitors via a GRP78-ATF6-CHOP axis
Article Snippet: The membranes were subsequently blocked with fast blocking buffer (GF1815, Beyotime, China) for 30 min at room temperature prior to antibody staining. .. Specific primary antibodies (a GRP78 polyclonal antibody (11587-1-AP-50UL), ATF6 polyclonal antibody (24169-1-AP-50UL), CHOP polyclonal antibody (15204-1-AP-50UL), phospho-EIF2S1 (Ser51) monoclonal antibody (68023-1-Ig), ATF4 polyclonal antibody (10835-1-AP), and beta-actin recombinant antibody (81115-1-RR) from Proteintech (USA), a phospho-IRE1 (Ser724) antibody (AF7150), XBP1 antibody (AF5110), and tubulin beta antibody (AF7011) from Affinity (USA), and a P-PERK antibody (T982, WL05295) from Wanleibio (Shengyang)) were added overnight incubation at 4 °C. .. A secondary antibody (HRP-conjugated AffiniPure goat anti-rabbit lgG (H + L) (SA00001-2-100UL) or HRP-conjugated AffiniPure goat anti-mouse lgG (H + L) (SA00001-1-100UL), both from Proteintech, USA) was then added, and incubated with the membrane for 1 h at room temperature.

Article Title: Targeting ceramide transfer protein sensitizes AML to FLT3 inhibitors via a GRP78-ATF6-CHOP axis.
Article Snippet: Themembranes were subsequently blocked with fast blocking buffer (GF1815, Beyotime, China) for 30min at roomtemperatureprior to antibody staining. .. Specific primary antibodies (a GRP78 polyclonal antibody (11587-1-AP-50UL), ATF6 polyclonal antibody (24169-1-AP50UL), CHOP polyclonal antibody (15204-1-AP-50UL), phospho-EIF2S1 (Ser51) monoclonal antibody (68023-1-Ig), ATF4 polyclonal antibody (10835-1-AP), and beta-actin recombinant antibody (81115-1-RR) from Proteintech (USA), a phospho-IRE1 (Ser724) antibody (AF7150), XBP1 antibody (AF5110), and tubulin beta antibody (AF7011) from Affinity (USA), and a P-PERK antibody (T982, WL05295) from Wanleibio (Shengyang)) were added overnight incubation at 4 °C. .. A secondary antibody (HRP-conjugated AffiniPure goat anti-rabbit lgG (H + L) (SA00001-2-100UL)orHRP-conjugatedAffiniPure goat anti-mouse lgG (H + L) (SA00001-1-100UL), both from Proteintech, USA) was then added, and incubatedwith themembrane for 1 h at room temperature.

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Article Title: Quantitative proteomic analysis identifies the unfolded protein response as a host pathway co-opted by ASFV to promote replication
Article Snippet: The viral titers were determined by performing the hemadsorption (HAD) assay, and the 50% HAD dose was calculated by using the method of Reed and Muench, as described previously , the HEK293T cells were obtained from the American Type Culture Collection (CRL-11268). .. The commercial antibodies used in this study included GRP78/BIP Rabbit Polyclonal Antibody (11587-1-AP, 1:1,000; Proteintech), Rabbit Anti-Phospho-PERK (Thr980) antibody (bs-3330R, 1:1,000; Bioss), PERK Rabbit pAb (A18196, 1:1,000; ABclonal), XBP1S-specific Polyclonal antibody (24868-1-AP, 1:1,000; Proteintech), Rabbit Monoclonal (EPR5253) to IRE1 (phosphoS724) ( AB124945 , 1:1,000; abcam), IRE1; ERN1 Polyclonal antibody (27528-1-AP, 1:1,000; Proteintech), ATF6 Polyclonal antibody (24169-1-AP, 1:1,000; Proteintech), Monoclonal Anti-Flag M2 antibody produced in mouse (F1804, 1:1,000; Sigma-Aldrich), Myc-Tag Rabbit mAb (C-terminal) (AE070, 1:1,000; ABclonal), MYC tag mouse monoclonal antibody (60003-2-1g, 1:1,000; Proteintech), Goat Anti-rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, AlexaFluor 488 (A-11008, 1:1,000; Thermo Fisher Scientific), Goat Anti-rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 (A11037, 1:1,000; Thermo Fisher Scientific), Goat Anti-mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 (A11005,1:1000, Thermo Fisher Scientific), Goat Anti-Mouse IgG (H + L) Superclonal Secondary Antibody, Alexa Fluor 488 (A28175, 1:1,000; Thermo Fisher Scientific), GAPDH mouse monoclonal antibody (TA802519M, 1:1,000; Origene), β-tubulin mouse monoclonal antibody (66240-1-1g, 1:1,000; Proteintech), and anti-β-actin monoclonal antibody (sc-8432, 1:1,000; Santa Cruz Biotechnology). .. Ceapin-A7 (HY-108434), 4μ8C (HY-19707), and GSK2606414 (HY-18072) were obtained from Med Chem Express (MCE, USA).

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APEC OMVs activate UPR signaling to induce ERS . A HD11 cells were treated with 50–200 µg/mL OMVs for 6 h, and the relative mRNA levels of UPR downstream genes ATF4 , CHOP , EDEM1 , ERdj4 , and ATF6 were detected by qPCR. ( n = 3) B HD11 cells were treated with OMVs (100 µg/mL) for 0–9 h, and qPCR was used to detect the relative mRNA levels of UPR downstream genes ATF4 , CHOP , EDEM1 , ERdj4 , and ATF6 . ( n = 3) C , D HD11 cells were treated with OMVs (100 µg/mL) for 0–9 h, western blotting was used to detect the expression of PERK, p-PERK, eIF2α, p-eIF2α, CHOP, IRE1, p-IRE1, and ATF6 proteins in cell lysates, and gray value analysis was performed using ImageJ software ( D ). ( n = 3). E , F HD11 cells were treated with OMVs (100 µg/mL) for 6 h in the absence or presence of 4-PBA (2 mM), western blot analysis was used to detect the expression of PERK, p-PERK, eIF2α, p-eIF2α, CHOP, IRE1, p-IRE1, and ATF6 proteins in cell lysates, and gray value analysis was performed using ImageJ software ( F ). ( n = 3); n represents three biological replicates; data points indicate independent culture systems. Bar charts display mean ± standard error of the mean (SEM). Two-way ANOVA with Sidak correction was performed (* p < 0.05, ** p < 0.01, *** p < 0.001).

Journal: Veterinary Research

Article Title: Outer membrane vesicles secreted by avian pathogenic Escherichia coli promote its survival within macrophages and systemic infection by inducing endoplasmic reticulum stress-mediated autophagy flux blockade

doi: 10.1186/s13567-025-01679-6

Figure Lengend Snippet: APEC OMVs activate UPR signaling to induce ERS . A HD11 cells were treated with 50–200 µg/mL OMVs for 6 h, and the relative mRNA levels of UPR downstream genes ATF4 , CHOP , EDEM1 , ERdj4 , and ATF6 were detected by qPCR. ( n = 3) B HD11 cells were treated with OMVs (100 µg/mL) for 0–9 h, and qPCR was used to detect the relative mRNA levels of UPR downstream genes ATF4 , CHOP , EDEM1 , ERdj4 , and ATF6 . ( n = 3) C , D HD11 cells were treated with OMVs (100 µg/mL) for 0–9 h, western blotting was used to detect the expression of PERK, p-PERK, eIF2α, p-eIF2α, CHOP, IRE1, p-IRE1, and ATF6 proteins in cell lysates, and gray value analysis was performed using ImageJ software ( D ). ( n = 3). E , F HD11 cells were treated with OMVs (100 µg/mL) for 6 h in the absence or presence of 4-PBA (2 mM), western blot analysis was used to detect the expression of PERK, p-PERK, eIF2α, p-eIF2α, CHOP, IRE1, p-IRE1, and ATF6 proteins in cell lysates, and gray value analysis was performed using ImageJ software ( F ). ( n = 3); n represents three biological replicates; data points indicate independent culture systems. Bar charts display mean ± standard error of the mean (SEM). Two-way ANOVA with Sidak correction was performed (* p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: Rabbit anti-ATF6 polyclonal antibody , Boster, Hubei, China , 1:2000.

Techniques: Western Blot, Expressing, Software

OMVs secreted by avian pathogenic Escherichia coli promote its survival within macrophages and systemic infection by inducing ERS-mediated autophagy flux blockade . APEC-secreted OMVs, upon uptake by HD11 cells, induce ROS accumulation and Ca 2+ release, triggering ERS and activating UPR pathways, including the PERK, IRE1, and ATF6 signaling branches, leading to the expression of stress-related factors such as GRP78/BiP and CHOP. The sustained activation of ERS inhibits autophagosome degradation and disrupts the acidic environment of lysosomes, thereby preventing autophagosomes from fusing with lysosomes and impairing the phagocytic clearance capacity of macrophages. Collectively, these abnormal conditions facilitate APEC survival within HD11 cells and enable immune evasion, ultimately promoting bacterial dissemination and systemic infection in the host.

Journal: Veterinary Research

doi: 10.1186/s13567-025-01679-6

Figure Lengend Snippet: OMVs secreted by avian pathogenic Escherichia coli promote its survival within macrophages and systemic infection by inducing ERS-mediated autophagy flux blockade . APEC-secreted OMVs, upon uptake by HD11 cells, induce ROS accumulation and Ca 2+ release, triggering ERS and activating UPR pathways, including the PERK, IRE1, and ATF6 signaling branches, leading to the expression of stress-related factors such as GRP78/BiP and CHOP. The sustained activation of ERS inhibits autophagosome degradation and disrupts the acidic environment of lysosomes, thereby preventing autophagosomes from fusing with lysosomes and impairing the phagocytic clearance capacity of macrophages. Collectively, these abnormal conditions facilitate APEC survival within HD11 cells and enable immune evasion, ultimately promoting bacterial dissemination and systemic infection in the host.

Article Snippet: Rabbit anti-ATF6 polyclonal antibody , Boster, Hubei, China , 1:2000.

Techniques: Infection, Expressing, Activation Assay

D117L activates three UPR pathways through targeting key UPR components. ( A ) Heatmap of ASFV proteins identified by proteomics analysis across infection. ( B ) PPI network analysis of the virus-host interaction. ( C ) HEK293T cells were transfected with vector or Flag-D117L for 24 h, and mRNA expression of Ddit3, Dnajc3, and Dnajb9 was detected by RT-qPCR. ( D ) HEK293T cells were transfected with vector, Flag-D117L, Flag-D117LΔ38-60, Flag-D117LΔ67-76, or Flag-D117LΔ96-117; each group was also co-transfected with ATF6-LUC and Renilla-TK. After 24 h post-transfection, luciferase activities were examined. ( E ) Mass spectrometry was performed to identify the interacting proteins of D117L and D117LΔ38-60, and the top 10 proteins were listed. ( F ) Kyoto Encyclopedia of Gene and Genomes pathway enrichment analysis of D117L interacting proteins. ( G and H ) Immunofluorescence analysis of the colocalization of Flag-tagged D117L (green) and Myc-tagged or endogenous HSPA5 (red) in iPAM cells. ( I ) Immunoprecipitation (IP) assay examining the interaction between HSPA5 and D117L. ( J and K ) IP assays assessing the interaction of D117L (WT) or D117LΔ38-60, D117LΔ67-76, and D117LΔ96-117 mutants with CANX or HSPA5 in HEK293T cells.Data are presented as mean + SD. PP values were calculated by a two-tailed unpaired t-test. ** P < 0.01, *** P < 0.001.

Journal: mBio

Article Title: Quantitative proteomic analysis identifies the unfolded protein response as a host pathway co-opted by ASFV to promote replication

doi: 10.1128/mbio.03242-25

Figure Lengend Snippet: D117L activates three UPR pathways through targeting key UPR components. ( A ) Heatmap of ASFV proteins identified by proteomics analysis across infection. ( B ) PPI network analysis of the virus-host interaction. ( C ) HEK293T cells were transfected with vector or Flag-D117L for 24 h, and mRNA expression of Ddit3, Dnajc3, and Dnajb9 was detected by RT-qPCR. ( D ) HEK293T cells were transfected with vector, Flag-D117L, Flag-D117LΔ38-60, Flag-D117LΔ67-76, or Flag-D117LΔ96-117; each group was also co-transfected with ATF6-LUC and Renilla-TK. After 24 h post-transfection, luciferase activities were examined. ( E ) Mass spectrometry was performed to identify the interacting proteins of D117L and D117LΔ38-60, and the top 10 proteins were listed. ( F ) Kyoto Encyclopedia of Gene and Genomes pathway enrichment analysis of D117L interacting proteins. ( G and H ) Immunofluorescence analysis of the colocalization of Flag-tagged D117L (green) and Myc-tagged or endogenous HSPA5 (red) in iPAM cells. ( I ) Immunoprecipitation (IP) assay examining the interaction between HSPA5 and D117L. ( J and K ) IP assays assessing the interaction of D117L (WT) or D117LΔ38-60, D117LΔ67-76, and D117LΔ96-117 mutants with CANX or HSPA5 in HEK293T cells.Data are presented as mean + SD. PP values were calculated by a two-tailed unpaired t-test. ** P < 0.01, *** P < 0.001.

Article Snippet: The commercial antibodies used in this study included GRP78/BIP Rabbit Polyclonal Antibody (11587-1-AP, 1:1,000; Proteintech), Rabbit Anti-Phospho-PERK (Thr980) antibody (bs-3330R, 1:1,000; Bioss), PERK Rabbit pAb (A18196, 1:1,000; ABclonal), XBP1S-specific Polyclonal antibody (24868-1-AP, 1:1,000; Proteintech), Rabbit Monoclonal (EPR5253) to IRE1 (phosphoS724) ( AB124945 , 1:1,000; abcam), IRE1; ERN1 Polyclonal antibody (27528-1-AP, 1:1,000; Proteintech), ATF6 Polyclonal antibody (24169-1-AP, 1:1,000; Proteintech), Monoclonal Anti-Flag M2 antibody produced in mouse (F1804, 1:1,000; Sigma-Aldrich), Myc-Tag Rabbit mAb (C-terminal) (AE070, 1:1,000; ABclonal), MYC tag mouse monoclonal antibody (60003-2-1g, 1:1,000; Proteintech), Goat Anti-rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, AlexaFluor 488 (A-11008, 1:1,000; Thermo Fisher Scientific), Goat Anti-rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 (A11037, 1:1,000; Thermo Fisher Scientific), Goat Anti-mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 (A11005,1:1000, Thermo Fisher Scientific), Goat Anti-Mouse IgG (H + L) Superclonal Secondary Antibody, Alexa Fluor 488 (A28175, 1:1,000; Thermo Fisher Scientific), GAPDH mouse monoclonal antibody (TA802519M, 1:1,000; Origene), β-tubulin mouse monoclonal antibody (66240-1-1g, 1:1,000; Proteintech), and anti-β-actin monoclonal antibody (sc-8432, 1:1,000; Santa Cruz Biotechnology).

Techniques: Infection, Virus, Transfection, Plasmid Preparation, Expressing, Quantitative RT-PCR, Luciferase, Mass Spectrometry, Immunofluorescence, Immunoprecipitation, Two Tailed Test

Activation of UPR facilitates ASFV replication. ( A ) PAM cells were treated with or without inhibitor 4-PBA (50 µM), followed by ASFV challenge at 0.1 MOI for 24 h; P72 and P54 proteins were detected by Western blotting; and tubulin was used as a loading control. ( B ) PAM cells were treated with or without inhibitors, 4μ8C (5 µM), GSK2606414 (0.5 µM), or Ceapin-A7 (5 µM), and then infected with ASFV at 0.1 MOI for 24 h. P72 and P54 proteins were detected by Western blotting. ( C ) PAM cells were transfected with siRNA-NC (negative control), siRNA-Eif2AK3, siRNA-ERN1, or siRNA-ATF6 for 24 h and then challenged with ASFV at 0.1 MOI. P72 and P30 proteins were detected by Western blotting at 24 h; GAPDH was used as a loading control. ( D ) Fluorescence analysis of ASFV replication in PAM cells pretreated with or without inhibitors, 4μ8C (5 µM), GSK2606414 (0.5 µM), or Ceapin-A7 (5 µM). ( E–L ) The antiviral activities of the inhibitors against ASFV mRNA transcription. Relative mRNA expression levels of ASFV genes B646L and CP204L in PAM cells were quantified. PAM cells were treated with or without inhibitors or transfected with siRNAs and then infected with GFP-ASFV at 0.1 MOI for 24 h. Flow cytometry was used to analyze the percentage of infected positive cells ( M and O ). Viral titers were measured by TCID 50 assay at 72 h ( N and P ). Data presented as means + SD. P values were calculated by a two-tailed unpaired t -test. * P < 0.05, ** P < 0.01, *** P < 0.001.

Journal: mBio

Article Title: Quantitative proteomic analysis identifies the unfolded protein response as a host pathway co-opted by ASFV to promote replication

doi: 10.1128/mbio.03242-25

Figure Lengend Snippet: Activation of UPR facilitates ASFV replication. ( A ) PAM cells were treated with or without inhibitor 4-PBA (50 µM), followed by ASFV challenge at 0.1 MOI for 24 h; P72 and P54 proteins were detected by Western blotting; and tubulin was used as a loading control. ( B ) PAM cells were treated with or without inhibitors, 4μ8C (5 µM), GSK2606414 (0.5 µM), or Ceapin-A7 (5 µM), and then infected with ASFV at 0.1 MOI for 24 h. P72 and P54 proteins were detected by Western blotting. ( C ) PAM cells were transfected with siRNA-NC (negative control), siRNA-Eif2AK3, siRNA-ERN1, or siRNA-ATF6 for 24 h and then challenged with ASFV at 0.1 MOI. P72 and P30 proteins were detected by Western blotting at 24 h; GAPDH was used as a loading control. ( D ) Fluorescence analysis of ASFV replication in PAM cells pretreated with or without inhibitors, 4μ8C (5 µM), GSK2606414 (0.5 µM), or Ceapin-A7 (5 µM). ( E–L ) The antiviral activities of the inhibitors against ASFV mRNA transcription. Relative mRNA expression levels of ASFV genes B646L and CP204L in PAM cells were quantified. PAM cells were treated with or without inhibitors or transfected with siRNAs and then infected with GFP-ASFV at 0.1 MOI for 24 h. Flow cytometry was used to analyze the percentage of infected positive cells ( M and O ). Viral titers were measured by TCID 50 assay at 72 h ( N and P ). Data presented as means + SD. P values were calculated by a two-tailed unpaired t -test. * P < 0.05, ** P < 0.01, *** P < 0.001.

Techniques: Activation Assay, Western Blot, Control, Infection, Transfection, Negative Control, Fluorescence, Expressing, Flow Cytometry, Two Tailed Test

Representative fluorescent images (each image is an overlay with DAPI nuclear staining) and quantitative CTCF values for HSP70, GRP78, p‐IRE1α, p‐PERK, p‐eIF2α, and ATF6 in GC1 cells across all groups (C, SAL, HSM, HSMSAL). Composite images were generated by merging individual fluorescence channels. Data are expressed as mean ± SD ( n = 3, 10 fields/group). Statistical analysis: one‐way ANOVA, followed by Sidak's post hoc test. (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; statistical analysis was performed using graphpad Software). Scale bar: 50 μm.

Journal: FEBS Open Bio

Article Title: The effect of salubrinal on the endoplasmic reticulum stress pathway in heat‐stressed spermatogonial cells in vitro

doi: 10.1002/2211-5463.70169

Figure Lengend Snippet: Representative fluorescent images (each image is an overlay with DAPI nuclear staining) and quantitative CTCF values for HSP70, GRP78, p‐IRE1α, p‐PERK, p‐eIF2α, and ATF6 in GC1 cells across all groups (C, SAL, HSM, HSMSAL). Composite images were generated by merging individual fluorescence channels. Data are expressed as mean ± SD ( n = 3, 10 fields/group). Statistical analysis: one‐way ANOVA, followed by Sidak's post hoc test. (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; statistical analysis was performed using graphpad Software). Scale bar: 50 μm.

Article Snippet: After that, cells were incubated with blocking serum for 30 min. HSP70 (Biorbyt, orb157591, 1 : 200), p‐eIF2α (Bioss, BS‐4842, 1 : 200), p‐PERK (Bioss, bs‐3330R, 1 : 200), p‐IRE1α (Biorbyt, orb157704, 1 : 200), ATF6 (Bioss, bs‐1634R, 1 : 200), and GRP78 (Bioss, bs‐1219R, 1 : 200) rabbit polyclonal primary antibodies were incubated overnight for approximately 16 h at 4 °C in a humidified chamber.

Techniques: Staining, Generated, Fluorescence, Software

Journal: FEBS Open Bio

Article Title: The effect of salubrinal on the endoplasmic reticulum stress pathway in heat‐stressed spermatogonial cells in vitro

doi: 10.1002/2211-5463.70169

Figure Lengend Snippet: Representative fluorescent images (each image is an overlay with DAPI nuclear staining) and quantitative CTCF values for HSP70, GRP78, p‐IRE1α, p‐PERK, p‐eIF2α, and ATF6 in GC2 cells across all groups (C, SAL, HSM, HSMSAL). Composite images were generated by merging individual fluorescence channels. Data are expressed as mean ± SD ( n = 3, 10 fields per group). Statistical analysis: one‐way ANOVA, followed by Sidak's post hoc test. (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; statistical analysis was performed using graphpad Software). Scale bar: 50 μm.

Techniques: Staining, Generated, Fluorescence, Software

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rabbit polyclonal anti atf6 (Proteintech)

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